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HighYield T7 AF594 RNA Labeling Kit

貨號(hào)	RNT-101-AF594	售價(jià)（元）	5475
規(guī)格	20T x 20 μl	CAS號(hào)

產(chǎn)品簡(jiǎn)介
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產(chǎn)品信息

品牌	貨號(hào)	名稱	規(guī)格	單價(jià)
Jena Bioscience	RNT-101-AF594	HighYield T7 AF594 RNA Labeling Kit	20T x 20 μl	5475.00

產(chǎn)品簡(jiǎn)介

HighYield T7 AF594 RNA標(biāo)記試劑盒設(shè)計(jì)用于通過體外轉(zhuǎn)錄隨機(jī)產(chǎn)生AF594修飾的RNA探針。這種探針非常適合原位雜交和Northern Blot實(shí)驗(yàn)。AF594（也稱為Alexa Fluor?594）是一種親水性染料，與德克薩斯紅相比，其光穩(wěn)定性更強(qiáng)。

UTP-PEG5-AF594使用優(yōu)化的反應(yīng)緩沖液和T7 RNA標(biāo)記聚合酶混合物有效地并入RNA中，作為其天然對(duì)應(yīng)物UTP的替代物。35%UTP-PEG5-AF594替代通常會(huì)在反應(yīng)和標(biāo)記效率之間實(shí)現(xiàn)最佳平衡。然而，UTP-PEG5-AF594/UTP比值的單獨(dú)優(yōu)化可以通過單核苷酸格式輕松實(shí)現(xiàn)。由此產(chǎn)生的AF594修飾RNA探針隨后可以通過熒光光譜檢測(cè)。

該試劑盒含有足夠的試劑，用于20個(gè)標(biāo)記反應(yīng)，每個(gè)反應(yīng)20μl（35%UTP-PEG5-AF594替代物、2.5 mM ATP、GTP、CTP、0.2 mM UTP、0.1 mM UTP-PEG-5-AF595）。培養(yǎng)30分鐘后，20μl反應(yīng)產(chǎn)生約5-7μg RNA（1μg T7對(duì)照模板，1.4 kb RNA轉(zhuǎn)錄物）。然而，產(chǎn)量可能因模板而異（啟動(dòng)子設(shè)計(jì)、序列長(zhǎng)度、二級(jí)結(jié)構(gòu)形成）。

Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

UTP-PEG5-AF594
1x 10 μl (5 mM)

T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)

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