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Invitrogen? MagniSort? 人 CD4 T 細胞 2-Step富集試劑盒

貨號	8802-6831-74	售價（元）	9126
規(guī)格	200T	CAS號

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產(chǎn)品簡介

MagniSort? 人 CD4 2 Step富集試劑盒設(shè)計用于在第一次消耗單核細胞后通過陽性篩選對 CD4+ T 細胞進行磁性分離。它已經(jīng)過優(yōu)化，可使用生物素化抗人 CD14 和抗人 CD4 抗體以及鏈霉素親和素包被磁珠從人外周血單核細胞中分離 CD4+ T 細胞。在第一步中，單核細胞被抗人 CD14 抗體結(jié)合，然后被磁珠結(jié)合。當(dāng)置于磁場中時，單核細胞固定在磁鐵中，而剩余的細胞則被倒出，用于第二步。第二步，消耗單核細胞的細胞被抗人 CD4 抗體結(jié)合，然后被磁珠結(jié)合。現(xiàn)在，當(dāng)置于磁場中時，CD4+ T 細胞固定在磁鐵中，而不需要的細胞則被倒出。

富集后，可通過用抗人 CD4、克隆 OKT4 或 SK3 染色來驗證所選細胞的純度。

已報告應(yīng)用
磁性細胞分離

For Research Use Only. Not for use in diagnostic procedures.

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